Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation
نویسندگان
چکیده
منابع مشابه
Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation
RNA interference-mediated gene silencing was shown to be an efficient tool for validation of targets that may become anti-tick vaccine components. Here, we demonstrate the application of this approach in the validation of components of molecular signaling cascades, such as the Protein Kinase B (AKT)/Glycogen Synthase Kinase (GSK) axis during tick embryogenesis. It was shown that heptane and hyp...
متن کاملCorrection: Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation
Fig 5, " Silencing of AKT and GSK changes glycogen content R. microplus eggs, " is incorrect. Please see the correct Fig 5 and its caption here. article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
متن کاملRapid Delivery of Gold Nanoparticles into Colon Cancer HT-29 Cells by Electroporation: In-vitro Study
Background: Electroporation has become a routine technique for rapid drug delivery for the treatment of cancer. Because of its simplicity and wide range of application, it has been applied for the transfer of gold-nanoparticles and can facilitate entry into target cancer cells. Objective: The aim of this study is finding optimal conditions in order to obtain high GNPs- uptake and cell via...
متن کاملIntroduction of foreign genes into silkworm eggs by electroporation and its application in transgenic vector test.
Electroporation as a methodology to introduce foreign genes into silkworm eggs was systematically analyzed. The foreign gene in both the newly hatched and 3rd instar larva DNA can be detected by PCR. The amount of foreign gene in 3rd instar larva DNA was about 1/1000 of that in newly hatched larva DNA. The ratio of foreign gene entering into silkworm eggs was voltage dependent and showed signif...
متن کاملFunctional genomics tool: Gene silencing in Ixodes scapularis eggs and nymphs by electroporated dsRNA
BACKGROUND Ticks are blood-sucking arthropods responsible for transmitting a wide variety of disease-causing agents, and constitute important public health threats globally. Ixodes scapularis is the primary vector of the Lyme disease agent in the eastern and central U.S. RNAi is a mechanism by which gene-specific double-stranded RNA (dsRNA) triggers degradation of homologous mRNA transcripts. H...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS ONE
سال: 2015
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0130008